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Enabling better global research outcomes in soil, plant & environmental monitoring.

Photosynthesis Yield Analyzer

The MINI-PAM-II fluorometer combines the merits of its predecessor “MINI-PAM” with updated LED and computer technology.
Sensitivity, small dimensions, reliability under rugged conditions, and simple execution of fluorescence analysis makes the MINI-PAM-II the new standard for PAM fluorometry in field research.
Well-tested fibreoptics with 5.5 mm or 2 mm active diameter reach even hidden samples.

Measurements under field conditions are easily controlled and monitored by a transflective touchscreen. Energy-efficient LED sources, storage capacity of 27,000 data sets, and easy replaceable off-the-shelf batteries permit long term research campaigns in remote locations.
A new fully digital leaf clip combines fluorescence analysis with measurements of photosynthetically active radiation (PAR), leaf temperature and relative humidity.

The MINI-PAM-II is expandable through accessories such as an external multicolour lamp, optical oxygen sensor and barcode scanner.

Optoelectronic Unit MINI-PAM-II/B (BLUE Version)

Measuring light: Blue (470 nm) LED, standard modulation frequencies 5 to 25 Hz adjustable in increments of 5 Hz and 100 Hz, measuring light PAR at standard settings = 0.05 μmol m-2 s-1. Fluorescence at wavelengths greater than 630 nm is measured
Actinic light: Same blue LED as for measuring light, maximum actinic PAR = 3000 μmol m-2 s-1, maximum PAR of saturation pulses = 6000 μmol m-2 s-1 adjustable at increments of 500 μmol m-2 s-1

Optoelectronic Unit MINI-PAM-II/R (RED Version)

Measuring light: Red (655 nm) LED, modulation frequencies and PAR as described for MINI-PAM-II/B. Fluorescence at wavelengths greater than 700 nm is measured
Actinic light: Same red LED as for measuring light, maximum PAR of actinic light and saturation pulses as described for MINI-PAM-II/B

Optoelectronic Unit MINI-PAM-II/B & MINI-PAM-II/R

Far red light: Peak emission at 735 nm
Signal detection: PIN photodiode protected by long-pass and a short-pass filters
Data memory: Flash memory, 8 MB, providing memory for more than 27000 saturation pulse analyses
Display: Backlit 160 x 104 dots (78 x 61 mm) transflective B/W LCD display with resistive touch screen
Ports: Ports for fibre optics, USB cable, external light source, 2035-B leaf clip, auxiliaries and 12 V DC power supply
Power supply: 6 AA (Mignon) rechargeable batteries (Eneloop 1.2 V/2 Ah), providing power for up to 1000 yield measurements; 6 spare batteries, automatic power/off, battery charger (100 to 240 V AC, 50-60 Hz, 0.35 A) for 1 to 8 AA/AAA NI-MH/NI-CD batteries, 12 V 5.5 A power supply MINI PAM-II/N
Operating temperature: -5 to +45°C (non-condensing)
Dimensions: 17.2 cm x 11.2 cm x 7.6 cm (L x W x H)
Weight: 1.5 kg (incl. batteries)

Fiberoptics MINI-PAM/F

Design: Randomised 70 μm glass fibres forming single plastic shielded bundle with stainless steel adapter ends
Dimensions: Active diameter 5.5 mm, outer diameter 8 mm, length 100 cm
Weight: 180 g

Power Supply MINI-PAM-II/N

Input: 100 to 240 V AC, 50 to 60 Hz
Output: 12 V DC, 5.5 A
Operating temperature: -5 to +45°C (non-condensing)
Dimensions: 13 cm x 5.5 cm x 3 cm (L x W x H)
Weight: 350 g including cables

Battery Charger 000190101101

Input: 100 to 240 V AC, 50 to 60 Hz
Output: 12 V DC, 1.0 A
Operating temperature: -5 to +45°C (non-condensing)
Dimensions: 17.5 cm x 10.5 cm x 3 cm (L x W x H)
Weight: 300 g including cable

Distance Clip 60° 2010-A

Design: Metal clip with fibre holder and 11 mm sample hole: 5.5 cm x 1.4 cm (L x W)

Complementary Items

Sloped Plexiglas rack for convenient desktop operation. Stylus for touch screen. Carrying strap for optoelectronic unit

Transport Case MINI-PAM-II/T

Design: Aluminium case with custom foam packing
Dimensions: 50 cm x 34 cm x 20 cm (L x W x H)
Weight: 3.8 kg

Software WinControl-3

Program: WinControl-3 System Control and Data Acquisition Program (Windows XP/Vista, Windows 7+8 32-bit and 64-bit) for operation of measuring system via PC, data acquisition and analysis
Saturation Pulse Analysis: Measured: Ft, F0, FM, F, F0’ (also calculated), FM’. PAR, leaf temperature and relative humidity using 2035-B Leaf-Clip Holder. Calculated: F0’ (also measured), FV/FM and Y(II) (maximum and effective photochemical yield of PS II, respectively), qL, qP, qN, NPQ, Y(NPQ), Y(NO) and ETR (electron transport rate)
Fitting Routines: Two routines for determination of the cardinal points α, Ik and ETRmax of light curves
Programmed Features: Automatic determination of signal offset for all light intensities and all gain levels. Automatic calibration of internal PAR sensor against an external PAR sensor connected to the MINI-PAM-II
Communication Protocol: USB
Computer Requirements: Processor: 0.8 GHz. RAM: 512MB. Screen resolution: 1024 x 600 pixels. Interface: USB 2.0/3.0

Microsecond timing enables the MINI-PAM-II fluorometer to use the same LED as a source for PAM measuring light, actinic light and saturation pulses. Measuring light corresponds to μs flashes of constant amplitude, actinic light is quasi-constant light employed to drive photosynthesis, and saturation pulses temporarily saturate primary photosynthesis so that all photosystem II reaction centres are “closed”.

Being a PAM fluorometer, the MINI-PAM-II device records only the fluorescence elicited by measuring light. Fluorescence excited by internal actinic light, saturation pulses or constant external light, like sun radiation, is not measured. Therefore, the MINI-PAM-II determines how environmental factors modulate the efficiency of conversion of measuring light into fluorescence. These “PAM fluorescence data” are required to retrieve information on primary photosynthesis like the photosynthetic efficiency of photosystem II, Y(II)

A second LED in the MINI-PAM-II emits far red light. This LED preferably excites photosystem I but is negligibly absorbed by photosystem II. A special measuring routine uses this far red LED to determine the F0’ fluorescence level which is important to correctly assess the reduction state of photosystem II reaction centers.

In experiments using internal actinic light, the light intensity at sample level can be monitored online using an internal light sensor. This internal sensor must be calibrated against an external light sensor.